Implementation of an In-line Surface-induced Dissociation Device in a Quadrupole Time-of-flight Instrument and Its Performance

Implementation of an In-line Surface-induced Dissociation Device in a Quadrupole Time-of-flight Instrument and Its Performance

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An ion funnel interface to improve ion transmission at high pressures was tested in a custom built quadrupole-surface-quadrupole instrument. The ion transmission efficiency for selected bio-molecules such as YGGFLR, insulin chain-B, ubiquitin and cytochrome c showed to approach almost 90%, with the funnel interface installed. The ion transmission efficiency was effected by several factors including: the size of the analyte, the DC gradient, the RF frequency, and the RF amplitude. The higher fragmentation efficiencies for SID in the presence of the funnel interface indicated higher internal energy deposition for the funnel interface.The higher fragmentation efficiencies for SID in the presence of the funnel interface indicated higher internal energy deposition for the funnel interface.


Title:Implementation of an In-line Surface-induced Dissociation Device in a Quadrupole Time-of-flight Instrument and Its Performance
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Publisher:ProQuest - 2008
ISBN-13:

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